Can I can set up multiple lists in Post Gopher?

Yes, you can!

The settings in your LeadGen settings in Post Gopher are the default settings applied to all the posts where you have activated Post Gopher.  However for each post you can modify these settings (on a post by post basis)

In the right hand menu of the Create/Edit post screen in your WordPress site you will find the option to change the default settings of Post Gopher for this post.  You may have to click the down-arrow to show these options